Fig. 4

T3 combined with control diet (CD) promoted browning of white adipose tissue (WAT) may through inhibiting of the PI3K/AKT signalling pathways: (A) Weight changes of HFD mice in first period (7 days) after HA or HA + T3 intervention; (B) Intake of HFD mice in first period (7 days) after HA or HA + T3 intervention; (C) Weight change of HFD mice in 8–21 days after HA or HA + T3 intervention under the condition of free diet (FD); (D) Weight change of HFD mice in 8–21 days after HA or HA + T3 intervention under the condition of CD; (E) Infrared thermography of basal surface on FD and FD + T3 mouse housed at 22 ℃; (F) Infrared thermography of basal surface on CD and CD + T3 mouse housed at 22 ℃; (G) HE staining of the liver in FD and FD + T3 groups; (H) HE staining of the liver in CD and CD + T3 groups; (I) The brown-related mRNA expression in WAT between FD and FD + T3 group; (J) THR and DIO₂ protein levels in WAT between FD and FD + T3 group; (K) The brown-related proteins in WAT between FD and FD + T3 group; (L) HE staining of WAT in FD and FD + T3 groups; (M) The protein expression levels of UCP-1 of WAT were detected by immunofluorescence in FD and FD + T3 groups; (N) The brown-related mRNA expression in WAT between CD and CD + T3 group; (O) THR and DIO₂ protein in WAT between CD and CD + T3 group; (P) The brown-related proteins in WAT between CD and CD + T3 group; (Q) HE staining of WAT in CD and CD + T3 groups; (R) The protein expression of UCP-1 of WAT were detected by immunofluorescence in CD and CD + T3 groups; (S) The expression levels of PI3K/AKT signalling pathway related proteins in WAT of CD and CD + T3 groups were measured using Western blot. (FD: free diet; CD: control diet) The height of each bar represents the mean ± SD for the group (B: *p < 0.05, **p < 0.01, ***p < 0.001 vs HA group, n = 10; K: ***p < 0.001 vs FD group, n = 3; J–N,S: *p < 0.05, **p < 0.01, ***p < 0.001 vs CD group, n = 3).