Fig. 5
From: Evaluation of survivorship and annulus validation in calcein-stained freshwater unionid mussels
Two images of the same thin-section from the 250 mg L−1 × 12-h treatment. The top image is of the slide illuminated by UV light at 490 nm with red arrows indicating where calcein can be seen to have etched at the growing edge and seeped into portions of the shell. The bottom image is of the slide under ambient light with three of the annuli indicated with red arrows. Annuli are indicated in both images by pencil marks. Individual was determined to be 20 years old.
