Fig. 7

Effect of DSF on S100A4⁺ macrophages in the lung tissues of BLM-induced lung fibrosis in mice. (A–C) Representative immunostaining images for S100A4 in the lung tissue of untreated (A), DSF non-treated (B) and DSF-treated (C) mice on day 28. Higher-magnification images in the lower left correspond to the areas indicated by dashed squares. Scale bars = 200 μm. (D) Representative bright-field and double-fluorescence staining images in the lung tissues of BLM-treated mice on day 28. DAPI (4′,6-diamidino-2-phenylindole) is shown in blue, CD68 in green, and S100A4 in red. The bright-field image was generated from fluorescent images using inForm software. Higher-magnification images correspond to the area indicated with dashed squares. Arrowheads indicate S100A4⁺CD68⁺ macrophages. Scale bars = 25 μm. (E) Number of S100A4⁺ cells in the lung tissue on day 28 counted in immunostaining images (A–C). n = 5 per group. *P < 0.05, **P < 0.01 (Tukey’s test). ns, not significant.