Fig. 4
Generation of macrophage-specific p38 MAPK knockout mice. (a) Macrophage-specific p38 MAPK knockout mice or control mice at 13–15 weeks of age were treated by intraperitoneal injection of CG or PBS three times a week for 3 weeks. (b) Expression of peritoneal Mapk14 mRNA in control or macrophage-specific p38 knockout mice with PBS or CG. Ctrl PBS; n = 6, p38ΔMΦ KO PBS; n = 6, Ctrl CG; n = 11, p38ΔMΦKO CG; n = 11. (c) Double immunofluorescence staining for p-p38 and F4/80 in control and macrophage-specific p38 MAPK knockout mice in PBS- or CG-treated mouse peritoneum. Triangles indicate double positive cells. High magnification of immunofluorescence staining for p-p38, F4/80 and DAPI in control and macrophage-specific p38 MAPK knockout mice in CG-treated mouse peritoneum. Ctrl; control mice, p38ΔMΦ KO; macrophage-specific p38 MAPK knockout mice. Scale bars represent 100 µm. Values are expressed as means ± SD. n.s.; not significant, **P < 0.01.
