Fig. 7
Knockdown of p38 MAPK suppressed mRNA expression of inflammatory cytokines induced by lipopolysaccharide in RAW 264.7 cells. (a) The cell culture protocol for transfection siRNA targeting Mapk14 (si-Mapk14) or negative control (si-NC) into RAW 264.7 cells. (b,c) Western blot analyses of phosphorylated p38 MAPK, total p38 MAPK, and β-actin in RAW 264.7 cells with or without p38 MAPK knockdown. Original blots/gels are presented in Supplementary Fig. S5. (d) Double immunofluorescence staining for phosphorylated p38 MAPK (p-p38) and F4/80 in RAW 264.7 cells. Scale bars represent 400 μm. (e) Expression of Il1b, Il6, Ccl2, and Il10 mRNA in RAW 264.7 cells with or without p38 MAPK knockdown. Values are expressed as means ± SD. **P < 0.01.
