Fig. 1 | Scientific Reports

Fig. 1

From: A colorimetric reverse-transcription loop-mediated isothermal amplification method targeting the L452R mutation to detect the Delta variant of SARS-CoV-2

Fig. 1

The fluorescence signal of the real-time RT-LAMP assay was used to amplify the RNA-positive Delta variant and wild-type strain (Wuhan-Hu-1) of SARS-CoV-2. The result was achieved using four primer sets with different temperatures. (a) The temperature for real-time RT-PCR amplification was set at 60°C. (b) The temperature for real-time RT-PCR amplification was set at 63°C. The primer set-0 (zero mismatch) is indicated as a red line color. The primer set-1 (one mismatch) is indicated as a yellow line color. The primer set-2 (two mismatches) is indicated as a purple line color. The primer set-3 (three mismatches) is indicated as a light blue line color. The NTC, or negative control, is indicated as a green color, meaning that all primer sets were tested with the wild-type strain (Wuhan-Hu-1) of SARS-CoV-2.

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