Fig. 3 | Scientific Reports

Fig. 3

From: A colorimetric reverse-transcription loop-mediated isothermal amplification method targeting the L452R mutation to detect the Delta variant of SARS-CoV-2

Fig. 3

Evaluation of representative clinical samples using the colorimetric RT-LAMP assay. The yellow color indicates a positive readout. The purple color indicates a negative one. Then, the RT-LAMP amplification products were observed on a 2%-agarose gel electrophoresis analysis. (a) The positive clinical sample results for the Delta variant of SARS-CoV-2 (D1–30) are represented by numbers 1–30. (b) The negative clinical sample results of SARS-CoV-2 (H1–30) are represented by numbers 1–30. P: Positive control (L452R-specific plasmid standard); N: Negative control (DNase/RNase-Free Distilled Water); L: 100 bp DNA ladder. The gel electrophoresis image was cropped to emphasize only the samples loaded in the gel, and the original gels are provided in Supplementary Figure S4–S7.

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