Fig. 4 | Scientific Reports

Fig. 4

From: A colorimetric reverse-transcription loop-mediated isothermal amplification method targeting the L452R mutation to detect the Delta variant of SARS-CoV-2

Fig. 4

Primer information for the RT-LAMP assay. (a) Schematic depiction of the RT-LAMP primer target location. The L452R mutation is a SNP located in the RBD of the spike gene within the SARS-CoV-2 genome. (b) Illustration demonstrating RT-LAMP primers designed to identify the L452R mutation in the Delta variant of SARS-CoV-2 using the sequence from GenBank: OP412303.1. The SNP of the L452R mutation is shown in the red box, changing nucleotide position 1355 from U to G (CTG to CGG), which generated 0–3 nucleotide base mismatches (pink highlighted) adjacent to the specific SNP position on F2 and LPF sequences. Primers F3, F2, B3, B2, F1c, B1c, and LPF and direction show primer sequence positions. (c) Schematic representation of the RT-LAMP primers used in this study. A FIP consisted of F1c and F2, and a BIP consisted of B1c and B2. The sequence of all primer sets is presented in Table 1.

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