Fig. 2 | Scientific Reports

Fig. 2

From: Subtracting the background by reducing cell-free DNA’s confounding effects on Mycobacterium tuberculosis quantitation and the sputum microbiome

Fig. 2

PMA and PEMAX reduce MTBC detection in dilution series (regardless of how aliquots were treated) and in sputum sediments from presumptive TB patients. PCR readouts for non-antibiotic (cultured in absence of antibiotics), antibiotic (cultured with isoniazid and rifampicin), 1:1 mixture (non-antibiotic: antibiotic), and heat-killed (non-antibiotic aliquots heated at 100 °C for 30 min) cultures after 24 h of incubation. Supplementary Fig. 1 has these data for the 48 h and 72 h exposure period. Median CTs (IQR) are shown for (A) 108 and (B) 106 CFU/ml dilutions. Open circles denote CTs that exceed those of the averaged non-template control (dashed line). Median Xpert CTmin (95% CI) are shown for (C) 108 CFU/ml and (D) 106 CFU/ml. Triangles on y-axes indicate decreasing bacterial load. Purple dots: untreated; red dots: PMA; green dots: PEMAX. MTBCMycobacterium tuberculosis complex, CT cycle threshold, CTmin minimum cycle threshold, CI confidence interval, IQR interquartile range.

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