Fig. 2
From: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries
Producing reducing sugar from phosphoric-acid swollen cellulose (PSC, 3.5 mg mL–1) in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C for 96 h in the presence of free CD, a CD and CBD mixture (CD + CBD), and a native modular enzymes (native) CD and CBD cluster on streptavidin-conjugated CdSe nanoparticles (hybrid cellulase complex), made from five module enzymes, (a) CD5-6, (b) CD6-4, (c) CD7, (d) CD26, and (e) CD44. Native CBDs used in the experiments were CBD1-4, CBD2-3, CBD1-5, CBD11 and CBD44, respectively. All experiments were performed at a CD concentration of 40 nM.
