Fig. 3
From: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries
Producing reducing sugar from 3.5 mg mL–1 of PSC in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C for 96 h in the presence of (a) CD5-6, (b) CD6-4, (c) CD7, (d) CD26, and (e) CD44, made from modular enzymes, and different CBD clusters on streptavidin-conjugated CdSe nanoparticles (hybrid cellulase complexes). Producing reducing sugar from PSC (3.5 mg mL-1) in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C for 96 h in the presence of (f) CD8-2, (g) CD9-3, (h) CD74, and (i) CD124, made from non-module enzymes, and different CBD clusters on hybrid cellulase complexes. All experiments were performed at a CD concentration of 40 nM. The free CD was used as a reference. N and NC show native enzymes and native clustering enzymes, respectively.
