Fig. 4
From: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries
The reducing sugar production from PSC (3.5 mg mL−1) in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C at 96 h with (a) CD6-4 (CD), a native module structure CD6-4-CBD2-3 (N) or hybrid cellulase complexes with native CBD (NC). (b) Reducing sugar production using a hybrid cellulase complexes with different CBDs (blue bar), (c) CD6-4-CBD46 and CD6-4-CBDx clusters on separate streptavidin-conjugated CdSe nanoparticles mixed at an equal molar ratio (1:1) (red bar), and with (d) CD6-4-CBM46, CD6-4-CBM2-5, and another CD6-4-CBDx cluster on separate streptavidin-conjugated CdSe nanoparticles (green bar) mixed at an equal molar ratio (1:1:1). All experiments were performed at a total CD concentration of 40 nM, and the CD/CBM ratios were 7:23. Each experiment was conducted thrice, and the average values are plotted with error bars representing standard variations.
