Fig. 6
From: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries
Producing reducing sugar from Avicel (10 mg mL-1) in a 50 mM acetate buffer (pH 5.0, 200 mM NaCl) at 45 °C for 96 h in the presence of (a) free CD6-4 (open black circles), native module enzyme (closed black circles), native clustering enzymes (open black square) (used as references). (b) Hybrid cellulase complexes with CD6-4 and different CBDs indicating the top five producing reducing sugar from PSC (blue symbols) of (c) two types (red symbols) and (d) three types (green symbols). All experiments were performed at a CD concentration of 2.5 μM.
