Fig. 8
From: Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries
Thermal stability of clustered enzymes (normal line) and free (dotted line) at 50 °C (blue) and 70 °C (red). The enzymes (2.5 μM) were maintained at both temperatures without the substrate for 3 days in acetate buffer at pH 5.0. The activity determined before incubation was at 100%. Produced reducing sugars were measured at an enzyme concentration of 40 nM. The actual reducing sugar concentrations produced by these hybrid cellulase complex solutions at 0 h were 0.57 mg ml-1 at 50 ℃ at without nanoparticles, 1.96 mg ml-1 at 50 ℃ with nanoparticles, 0.55 mg ml-1 at 70 ℃ without nanoparticles, and 1.87 mg ml-1 at 70 ℃ with nanoparticles.
