Fig. 3

Differentiated pECs have angiogenesis function. (A) Tube formation images in HUVECs, SUVECs, and differentiated pECs on Matrigel for 4 h. Scale bar = 500 µm. (B) Branch points of HUVECs, SUVECs, and differentiated pECs. (n = 3) (C) Spheroid sprouting images of HUVECs, SUVECs, and differentiated pECs embedded in collagen gels. Phalloidin (red) stained cytoskeleton for sprouting measurement. The nucleus was stained with DAPI (blue). Scale bar = 275 µm. (D) Sprouts length was measured by ImageJ. (n = 4) Values presented as mean SEM. * P < 0.05, ** P < 0.01.