Fig. 5 | Scientific Reports

Fig. 5

From: Angiogenic properties and intercellular communication of differentiated porcine endothelial cells in vascular therapy

Fig. 5The alternative text for this image may have been generated using AI.

GAP junction formation between porcine and human ECs. (A) Immunofluorescence image illustrating connexin 43 (green) in HUVECs and SUVECs, with SUVECs stained using PKH 26 (red). Nuclei was stained with DAPI (blue). (B) Immunofluorescence depicting connexin 43 (green) in HUVECs and differentiated pECs, with differentiated pECs stained using PKH 26 (red). Nuclei was stained with DAPI (blue). (C) Western blotting data for co-IP showing the interaction between Myc-pCx43 and HA-hCx43 in HEK293T cells transfected with Myc-pCx43 and HA-hCx43. (D) Fluorescence image displaying small nucleotide tagged Cy3 (red) in SUVECs, transported by connexin channels from HUVECs. (E) Fluorescence image showing small nucleotide tagged Cy3 (red) in differentiated pECs, transported by connexin channels from HUVECs. Scale bar = 20 µm.

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