Table 4 Ct value of candidate reference genes.

From: Mining and expression analysis of color related genes in Bougainvillea glabra bracts based on transcriptome sequencing

Reference gene

Ct value

Mean

Standard deviation

Coefficient of variation /%

A

C

E

F

G

atp1

16.98

16.49

17.95

16.34

17.00

16.95

0.56

0.03

26 S

18.57

18.48

19.68

17.85

19.35

18.79

0.65

0.04

18 S

10.59

11.95

11.09

9.85

11.07

10.91

0.69

0.06

  1. The Ct value represents the number of cycles at which the fluorescence signal reaches the threshold (the threshold fluorescence signal is usually 10 times the baseline fluorescence signal); CV = (standard deviation/mean) * 100%.