Fig. 2 | Scientific Reports

Fig. 2

From: Local stimulation of pyramidal neurons in deep cortical layers of anesthetized rats enhances cortical visual information processing

Fig. 2

Virus injections and recordings were made in the deep layers of the visual cortex area V1. (A) A schematic representation of cortical coronal section containing area V1. (B) A brain section from a rat injected with the CaMKII virus. The gray dashed polygonal line indicates the recording site marked by making a lesion by passing current at the end of the recording session. Note the long fluorescent apical dendrites labeled by virus-delivered GFP; they are shown at higher resolution in the inset on the left, and the white arrowheads indicate these dendrites. (C) A brain section from a rat injected with the PV virus. Note the absence of fluorescent apical dendrites. (D) An example of a neuron labeled with the CaMKII virus that delivered GFP (green) costained with a red fluorescent probe by employing immunohistochemistry for the CaMKIIa antigen. (E) A microphotograph of a tetrode with an optical fiber attached. Abbreviations: SC, superior colliculus; V1, V2, areas V1 and V2; HIPP, hippocampus.

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