Fig. 1

Expressions of ladinin-1 (LAD1) in oral squamous cell carcinoma (OSCC) cell lines, HSC-2, HSC-3, and HSC-4. (a) Immunofluorescence for LAD1 (green) and DAPI (blue); scale bars: 50 μm. (b) LAD1 mRNA expression via real-time polymerase chain reaction (real-time PCR). (c) Protein expression using western blotting. LAD1 was localized in a linear pattern in the periphery of HSC-2, HSC-3, and HSC-4 cells, and such peripheral linear signals were most enhanced when they formed round clusters in HSC-2 and HSC-3 cells (a). The three SCC cell lines showed similar gene (b) and protein (c) expression levels for LAD1.