Fig. 4
Knockdown of SLC45A4 reduced glucose utilization, ATP, and lactic acid production in EOC cells, and inhibited the reprogramming of glycolytic energy metabolism. Transfected EOC cells with SLC45A4 knockdown. (a) Shows the differential gene volcano map, histogram and GSEA enrichment analysis between control group and SLC45A4 knockdown group. (b) KEGG pathway enrichment analysis and GO enrichment analysis between control group and SLC45A4 knockdown group. (c) Glucose uptake. (d) Lactic acid production. (e) The production of ATP.(f) Western blot verification of HIF-1α pathway. (g) The expression of PKM2 decreased after SLC45A4 knockdown in SKOV3 cells by immunofluorescence assay.(h) The expression of HK2 in OVCAR3 cells decreased after SLC45A4 was knocked down by immunofluorescence assay. The scales are 58 μm. (i) The expression levels of key glycolytic enzymes PKM2 and HK2 were different between control group and SLC45A4 knockdown group.
