Figure 6
From: The syntaxin-binding protein STXBP5 regulates progerin expression
RNA-seq analysis of the effects of STXBP5 knockdown or overexpression on HA-progerin-expressing HEK293 cells. The upregulated (A) or downregulated (B) DEGs in HA-progerin-expressing HEK293 cells overexpressing STXBP5 (relative to those overexpressing the empty vector) were identified through KEGG analysis. The upregulated (C) or downregulated (D) DEGs in the gSTXBP5 (STXBP5 guide3) group compared with the gAAVS1 group of HA-progerin-expressing HEK293 cells were identified through GO term and KEGG pathway enrichment analysis. (E,F) Heatmap of SASP related genes and SASP pathway genes that were differentially expressed between empty vector-overexpressing and STXBP5 overexpressing HA-progerin-expressing HEK293 cells. (G,H) Heatmap of SASP-related genes and SASP pathway genes differentially expressed between the gAAVS1 and gSTXBP5 groups of HA-progerin-expressing HEK293 cells. Red indicates genes that behaved differently than expected. Heatmaps were created with ggplot2 (version: 3.4.4, https://cran.r-project.org/web/packages/ggplot2). In all heatmaps, the p-value < 0.05; genes are represented in columns and technical replicates are represented in rows; the colour intensity of the Z-score column represents higher (red) to lower (blue) expression. (I) Scatter plot of TE expression in the STXBP5 OE and (J) gSTXBP5 groups. The coloured dots indicate TEs whose expression changed significantly (adjusted p-value < 0.05). In all the bubble charts, the dot colour indicates the level of statistical significance (-log10 (p-value)) and the dot size indicates the number of genes in the corresponding GO or KEGG term.
