Table 3 Total content and qualitative composition of polyphenols (µg/g) in shortcrust pastry cookies enriched with bee products determined by HPLC-DAD-MS.

From: Enrichment of shortcrust pastry cookies with bee products: polyphenol profile, in vitro bioactive potential, heat-induced compounds content, colour parameters and sensory changes

Compounds

Shortcrust pastry cookies

Control

Bee bread addition

Bee pollen addition

C

3%

5%

10%

3%

5%

10%

Phenolic acids

 Chlorogenic acid

2.33 ± 0.06c

2.10 ± 0.03c

2.05 ± 0.01c

2.33 ± 0.02c

3.19 ± 0.01b

3.88 ± 0.07a

3.94 ± 0.13a

 p-coumaric acid

nd

35.71 ± 0.01c

35.84 ± 0.04c

36.18 ± 0.05b

35.50 ± 0.01d

35.77 ± 0.03c

36.65 ± 0.08a

 Syringic acid

3.00 ± 0.04f

4.50 ± 0.05cd

4.65 ± 0.09c

3.90 ± 0.05e

5.44 ± 0.08a

4.25 ± 0.01d

5.17 ± 0.08b

 Benzoic acid

nd

nd

nd

14.71 ± 0.25c

nd

17.69 ± 0.14b

37.79 ± 0.23a

 Ferulic acid

63.26 ± 0.02e

63.47 ± 0.03cd

63.55 ± 0.06c

63.78 ± 0.03b

63.32 ± 0.02de

63.80 ± 0.04b

64.05 ± 0.05a

Flavonoids

 Quercetin

nd

58.53 ± 0.06c

60.06 ± 0.13b

63.57 ± 0.13a

58.27 ± 0.01c

60.01 ± 0.10b

63.98 ± 0.07a

 Rutin

19.67 ± 0.01f

23.63 ± 0.04e

25.65 ± 0.06d

32.15 ± 0.05c

32.32 ± 0.08c

44.37 ± 0.05b

67.20 ± 0.04a

 Naringenin

nd

64.01 ± 0.03f

65.15 ± 0.03e

67.45 ± 0.05d

66.66 ± 0.03c

69.59 ± 0.05b

75.37 ± 0.11a

 Apigenin

nd

nd

5.26 ± 0.02b

5.64 ± 0.08a

nd

nd

nd

 TPI

88.27 ± 0.14g

251.94 ± 0.26f

262.21 ± 0.08e

289.72 ± 0.51d

264.71 ± 0.07c

299.36 ± 0.19b

354.09 ± 0.71a

  1. The results are expressed as the mean ± SD. Different letters depict statistically significant differences (p ≤ 0.05) in the same row. C – control samples (cookies without the addition of bee products); BB – cookies enriched with bee bread; BP – cookies enriched with bee pollen. The percentages (3%, 5%, 10%) denote the proportion of bee bread or bee pollen incorporated into the cookies. nd – not detected; TPI- Total phenolic index calculated by the sum of individual phenolics identified in the tested samples.