Figure 3
From: Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor
(A) Determination of pronucleus formation time. (B) Number of pronuclei: a - unfertilized (white arrow: first polar body; yellow arrow: oocyte nucleus); b - diploid; c and d - polyploid. (C) Polyspermy-related gene expression. Bars with different letters within the same indicator denote significant differences at P < 0.05. The results are presented as the mean ± standard error of the mean (SEM) from at least three independent experimental replicates. PN: pronucleus; Melatonin: 10⁻⁹ M Melatonin; Mtn + Lu: 10⁻⁹ M Melatonin + 10⁻⁹ M Luzindole; Mtn + 4P: 10⁻⁹ M Melatonin + 10⁻⁹ M 4-P-PDOT.
