Table 4 Primer sequences utilized for real-time PCR.
From: Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor
Gene | Primer sequences (5’- 3’) | Product size (bp) | GenBank accession number |
|---|---|---|---|
GAPDH | F: GTCGGTTGTGGATCTGACCT R: TTGACGAAGTGGTCGTTGAG | 207 | NM_001206359 |
RN18s | F: TCCAATGGATCCTCGCGGAA R: GGCTACCACATCCAAGGAAG | 149 | NR_046261 |
JUNO | F: CTGACTGCCGCACATCCTAC R: GATCTTCTCGCACAGGTCGG | 146 | XM_013979283 |
CD9 | F: ATCCACTATGCCGCTGGACTG R: GGCTTCATTGGGAGGCTTGA | 229 | NM_214006 |
ZP2 | F: GAGTGACTCGCTACTCGCAA R: GTCACAGGATCTGCCACACA | 124 | NM_213848 |
ZP4 | F: GACCCACCCTGGACCTCTTA R: GCTCCCGAAGCAATTTCACC | 107 | NM_214045 |
RAB3A | F: GCCTTTGTCAGCACTGTAGGA R: ATAGGCTGTCGTGATGGTCC | 126 | NM_001123179 |
ASTL | F: GTGCTCTCCAGCAAGTACGA R: CCCAGACATGGGTACGATGG | 138 | XM_003481133 |
PAQR3 | F: TATGTGATCGCTGTCCTCGC R: GACCGCAAGGATGTGCCATA | 117 | XM_003129372 |
RAB8A | F: TGGCGAAGACCTACGATTACC R: GAGAAGCGGAACAGGACACA | 85 | NM_001243802 |
RCN1 | F: CCTCCCGCAAGACTATGACC R: AGCTTGGCTCCCAACAAACA | 133 | XM_003353917 |
CHOP | F: AGGCCTGGTATGAGGACCTG R: GCTGTGCCACTTTCCTTTCA | 339 | NM_001144845 |
PGC1α | F: TTCCGTATCACCACCCAAAT R: ATCTACTGCCTGGGGACCTT | 137 | NM_213963 |
TFAM | F: TCCGTTCAGTTTTGCGTATG R: TTGTACACCTGCCAGTCTGC | 240 | NM_001130211 |
POLG | F: ATGGGCCTTACAACGAGGTG R: GGCCTTCCTCATCAAAGGCT | 300 | XM_001927064 |
PRDX2 | F: TGTCCTTCGCCAGATCACT R: TCCACGTTGGGCTTGATT | 156 | NM_001244474 |
NRF1 | F: TTCTGCTGTGGCTGATGGAG R: AGTGCCATGGTGACTGTAGC | 174 | XM_021078993 |
TFB1M | F: TGGCAGAACCCAGATGACAC R: GGGCCATGATGGAAAGACGA | 101 | NM_001128475 |
