Table 1 Identification of spa typing and High Resolution Melting curve (HRM) (Tm values) among S. aureus strains isolated from milk samples.

From: Characterization of Staphylococcus aureus isolated from milk samples for their virulence, biofilm, and antimicrobial resistance

Sl. No

spa type

Amplicon size bp

Repeat type

HRM Tm

Dairy farm

Local vendor

Retail outlet

Total

1

t605

180

07-23

78.91 °C

8(22%)

4(14%)

6(25%)

18

2

t6296

320

07-23-21-22-34-12-23

79.85 °C

12(32%)

11(39%)

7(29%)

30

3

t267

400

07-23-12-21-17-34-34-34-33–34

79.31 °C

12(32%)

3(11%)

8(33%)

23

4

t1200

220

07-23-34

80.34 °C

1(3%)

4(14%)

2(8%)

7

5

Multiple bands

500

21-17-34-12-16-34-12-16

81.16 °C

4(11%)

6(21%)

1(4%)

11

Total

37

28

24

89