Fig. 3 | Scientific Reports

Fig. 3

From: Oxygen control in bioreactor drives high yield production of functional hiPSC-like hepatocytes for advanced liver disease modelling

Fig. 3

STB_4%O2 condition shows superior hepatic differentiation. (a) Relative gene expression of hepatic specific markers (HNF4a, AFP, ALB, A1AT, CYP3A4) in bioreactors operated under physiological (STB_4%O2, blue) and atmospheric (STB_21%O2, grey) levels of oxygen. (b) Immunofluorescence staining of HLC whole aggregates for ALB (red) and AFP (green) hepatic markers in both culture conditions. Scale bar: 100 μm. (c) Flow cytometry analysis of hepatocyte markers ALB and AFP, performed at the last day of differentiation (day 21) in STB_4%O2 (blue) and in STB_21%O2 (grey), PHH, primary human hepatocytes; ALB, Albumin; AFP, Alpha-fetoprotein. Data mean ± SEM. p ≤ 0.05(*), p ≤ 0.01 (**), p ≤ 0.0001 (****). nSTB_4%O2 =3, nSTB_21%O2 =3.

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