Fig. 5 | Scientific Reports

Fig. 5

From: Oxygen control in bioreactor drives high yield production of functional hiPSC-like hepatocytes for advanced liver disease modelling

Fig. 5

Characterization of the glyoxylate metabolism in hiPSC.HLC using the optimized process (STB_4%O2). (a) Simplified glyoxylate metabolism in PH1 disease at the cellular level. AGXT, alanine glyoxylate aminotransferase. (b-c) Specific secretion rates of glyoxylate (b) and oxalate by PH1.HLC (blue bars), ChiPSC18.HLC (green bars) (d) Intracellular Oxalate levels in PH1-HLC (blue), ChiPSC18.HLC (green). (e) AGXT gene expression in PH1.HLC (blue) and ChiPSC18.HLC (green) by qRT-PCR quantified using the 2−ΔΔCT method relatively to day 0 of differentiation and normalized for GAPDH and 36B4 housekeeping genes. Data shown represents mean ± SEM. p ≤ 0.05(*), p ≤ 0.01 (**), p ≤ 0.001 (***). nPH1.HLC=3, nChiPSC18.HLC=2.

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