Fig. 3
From: Tumor organoids improve mutation detection of pancreatic ductal adenocarcinoma
Validation of driver mutations captured by organoids but missed by bulk tumor WES. Targeted panel sequencing (target-seq) was performed to verify the presence of driver mutations that were detected in organoid WES but not tumor WES in three patients. (A) Procedure of sample preparation for target-seq. (B) Validation of driver mutations detected in organoids but missed by tumor using target-seq. Out of the seven driver mutations detected in organoids WES, target-seq confirmed four mutations as true somatic mutations. These mutations are shown with gene SYMBOL, ENSEMBL gene ID, and protein sequence name. VAF = Variant allele frequency.
