Fig. 2 | Scientific Reports

Fig. 2

From: A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate

Fig. 2

Quantitative amplification of HBV DNA with the effect of 2-Mercaptoethanol (2-ME) on Sodium dodecyl sulfate (SDS) surfactant. Quantitative amplification by qPCR of HBV DNA was performed on paired serum and DBS samples with the addition of different 2-ME concentration (0.1 to 0.4%) on the SDS surfactant. The HBV viral load is expressed as copies per milliliter on the y-axis, while surfactant concentrations, ranging from 0.2 to 1%, are presented on the x-axis. The red line corresponds to the Prime Direct Probe Taq measurement, while the blue line represents the Fast Advance Taq measurement. Circular and triangular markers indicate results for the first and second samples, respectively.

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