Fig. 10 | Scientific Reports

Fig. 10

From: Adipocytes reprogram the proteome of breast cancer cells in organotypic three-dimensional cell cultures

Fig. 10

APOB silencing impacts organotypic 3D culture formation. (A) qRT-PCR assays for control, mock, scramble, and siRNA-APOB transfected cells. (B) Optical microscopy (20X) images show the morphology, number, and size of the 3D structures of Hs578t cells grown over ECM-enriched geltrex substrate after silencing APOB and co-culturing with adipocytes for 48 h. (C-D) Quantification of the number and diameter of 3D cell structures showed in B. (E) Optical microscopy images showing and quantification of lipids using oil-red O of Hs578t cells grown in 3D conditions after silencing of APOB. (F) Quantification of oil-red O in cells showed in panel E. (G) Optical microscopy images showing and quantifying lipids using oil-red O in adipocytes cocultured with Hs578t cells after silencing of APOB. (F) Quantification of oil-red O stain of cells showed in panel G. *p < 0.05, **p < 0.01, ***p < 0.001, ns = no significant.

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