Fig. 3
Coculture of adipocytes with organotypic 3D culture of Hs578t breast cancer cells. (A) On-top 3D cell cultures of Hs578t cells. Optical microscopy images (40x) showing the typical morphology and 3D structures of Hs578 cells grown over ECM-enriched Geltrex substrate over time (0–6 days). The different cell densities used in cultures are indicated on the left. (B) Set up of 3D cell culture of Hs578t cells and adipocytes. (C) Optical microscopy images showing the Oil Red O staining of mature adipocytes (day 11) after incubating with Hs578 cells cultured in 3D for 48 h. Inset indicates a zoom imaging of a single adipocyte. (D) Quantification of lipids using Oil Red O staining in 3T3-L1 fibroblasts, mature adipocytes alone, and adipocytes after 48 h incubation with Hs578t cells. ***p < 0.001. ns = no significant.
