Fig. 3
Certain circRNA molecules involved in the regulation of BMSC osteogenic differentiation. (a) qPCR detection of the expression level changes of circRNAs screened by bioinformatics analysis after osteogenic differentiation induction. (b) qPCR detects the expression level changes of miRNAs screened by bioinformatics analysis after osteogenic differentiation induction. (c) qPCR detects the expression level changes of hsa_circ_0001600 and hsa-miR-542-3p during osteogenic differentiation at 0 days, 3 days, and 7 days. (d) qPCR detects the expression level changes of hsa_circ_0005991 and hsa-miR-424-5p during osteogenic differentiation at 0 days, 3 days, and 7 days. (e) Transfection of hsa_circ_0001600 expression in hBMSCs at a siRNA concentration of 50 nM for 48 h. (f) qPCR detects the changes in hsa-miR-542-3p levels in the si-hsa_circ_0001600 2 days after transfection. Compared with the si-NC, the expression of hsa-miR-542-3p increased in the si-hsa_circ_0001600. (si-hsa_circ_0001600 is the hsa_circ_0001600 siRNA transfected knockdown group, si-NC is the negative control group; * represents P < 0.05; ** represents p < 0.01; *** represents p < 0.001, **** represents p < 0.0001).
