Fig. 1 | Scientific Reports

Fig. 1

From: MicroRNA-130a-3p regulates osimertinib resistance by targeting runt-related transcription factor 3 in lung adenocarcinoma

Fig. 1

Characteristics of EVs secreted by PC9 and PC9OR cells in the medium (a) TEM images of isolated EVs from PC9 and PC9OR cells. The black sphere image shows 40-nm gold colloids bound to the CD9 antibody. Scale bars:100 nm. (b) Protein concentrations of CD9 and CD81 (exosomal markers) in isolated EVs from PC9 and PC9OR cells. Original blots are presented in Supplementary Figure S4a. These images show that EVs were isolated (a, b). (c) Size distribution of isolated EVs from PC9 and PC9OR cells. (d) Protein concentrations of isolated total EVs from PC9 and PC9OR cells. (e) Number of CD63- and CD9-positive EVs from PC9 and PC9OR cells. The release of EVs differed in the PC9 and PC9OR cells (c–e). Data are presented as mean ± SEM (n = 3–4) (d, e). *P < 0.05, compared with PC9 cells. P-values were determined using Student’s t-test. EVs, extracellular vesicles; TEM, transmission electron microscopy.

Back to article page