Fig. 2 | Scientific Reports

Fig. 2

From: MicroRNA-130a-3p regulates osimertinib resistance by targeting runt-related transcription factor 3 in lung adenocarcinoma

Fig. 2

Effects of PC9OR-EV- and PC9OR-EV-derived miRNAs on osimertinib resistance in PC9 cells (a) Fluorescence images of PC9 cells after exposure to red-labeled EVs from PC9 (PC9-EVs) and PC9OR cells (PC9OR-EVs). Scale bars: 50 μm. (b) Viability of PC9 cells treated with 5 µg of PC9-EVs and PC9OR-EVs in the absence or presence of 1 µM of osimertinib for 72 h. The controls are PC9 cells treated with PBS (vehicle). (c) The total concentration of miRNA derived from PC9-EVs and PC9OR-EVs. (d) Viability of PC9 cells transfected with 6–12 nM of miRNAs derived from PC9OR-EVs in the absence or presence of 1 µM osimertinib for 72 h. Data are presented as mean ± SEM (n = 3–6) (b–d). *P < 0.05, compared with control or PC9 cells. P-values were determined using Student’s t-test (c) or ANOVA with Tukey–Kramer (b, d). ns, no significant difference. EVs, extracellular vesicles; ANOVA, analysis of variance.

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