Fig. 3 | Scientific Reports

Fig. 3

From: MicroRNA-130a-3p regulates osimertinib resistance by targeting runt-related transcription factor 3 in lung adenocarcinoma

Fig. 3

Identification of EV-miRNAs associated with osimertinib resistance in lung adenocarcinoma cell lines (a) Scatter plot for the miRNA array analysis of isolated EVs from PC9 and PC9OR cells. (b) A heat map illustrating the expression of 22 EV-miRNAs and demonstrating a 3.5-fold change between PC9 and PC9OR cells. (c) Relative EV-miRNA expression levels in PC9 and PC9OR cells. (d) Relative EV-miRNA expression levels in H1975 and H1975OR cells. (e) Relative intracellular miRNA levels in PC9 and PC9OR cells. miRNA levels were normalized using U6 levels determined by RT-qPCR (c–e). Data are presented as mean ± SEM (n = 5–6). *P < 0.05, compared with PC9 or H1975 cells. P-values were determined using Student’s t-test. EVs, extracellular vesicles; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

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