Fig. 4
From: Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites

Bar plot showing the relative abundances of the plasmids of 11 species. Plasmid concentration at (a) 20 ng/µl and (b) 2 ng/µl. Bottom, no restriction enzyme treatment was performed; middle, the 11 plasmids were pooled first and then treated with the restriction enzyme, Nco1; top, each plasmid was first treated with the restriction enzyme (Nco1) and then pooled.