Table 2 Changes in PCR amplicon total amounts (ng) on individual parasite plasmids with various annealing temperatures.
From: Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites
Parasite/Ta (℃) | 45 | 50 | 55 | 65 | 70 |
|---|---|---|---|---|---|
Ascaris lumbricoides | 9.5 | 18.7 | 57 | 144 | 145 |
Clonorchis sinensis | 50 | 53 | 106.5 | 98.5 | 144 |
Dibothriocephalus latus | 40.55 | 57 | 70 | 121 | 77.5 |
Entamoeba histolytica | 11.55 | 17.1 | 52.5 | 45.9 | 44.6 |
Enterobius vermicularis | 11.9 | 24.95 | 68 | 150.5 | 150 |
Fasciola hepatica | 9.95 | 38.45 | 73.5 | 51 | 8.3 |
Giardia intestinalis | 16.4 | 14.65 | 38.4 | 45.1 | 34.45 |
Necator americanus | 11.1 | 23.7 | 66 | 105 | 71.5 |
Paragonimus westermani | 20.35 | 29.4 | 98 | 165.5 | 93.5 |
Taenia saginata | 17.5 | 26.35 | 39.95 | 158 | 85 |
Trichuris trichiura | 40.3 | 55.5 | 83 | 126.5 | 142.5 |
