Fig. 6
Examination of miR143#12 target genes in the rat AMI model. (A) Western blot analysis of COX-1, COX-2, and ATG7, which were identified as target genes of miR143#12 (#12) in the in vitro experiment and others in tissue samples from AMI rats. Tissue sampling was performed 24 h after AMI. The protein samples were extracted from the typical infarct and non-infarct tissues in each case of control miRNA (9 mg/kg) or miR143#12 (9 mg/kg) treated group. Western blot analysis of GAPDH in H2O2-exposed H9C2 cells was shown as a reference. (B) Band intensities with the same amount of protein loaded were evaluated by a densitometric analysis and the expression ratio of the infarct to non-infarct area in each protein was evaluated by the intensity value in each case. The ratios in miR143#12 cases were compared to those in control cases, which were defined as 1 (n = 4–7 in each). (C) The ratios of the mRNA expression levels of COX-1 or COX-2 to GAPDH evaluated by qRT-PCR in the same samples as in (A) were also shown. The ratios in miR143#12 cases were compared to those in control cases, which were defined as 1 (n = 4–7 in each). Results are shown as the mean ± SE. *p < 0.05, **p < 0.01, ***p < 0.001; 2-tailed, unpaired Student’s t test.
