Fig. 3
From: DTL promotes the growth and migration of melanoma cells through the ERK/E2F1/BUB1 axis
DTL depletion suppresses oncogenicity of melanoma cells. ((A−C) and (a−c)) The depleted expression of DTL was validated by appropriate fluorescence expression (C and c), qPCR (A and a) and western blot analysis (B and b). ((D–F) and (d–f)) Depleted expression of DTL inhibited the proliferative capability of melanoma cells by CCK8 proliferation assay (D and d), colony formation (E and e) assay and EdU staining (F and f). ((G–I) and (g–i)) Depleted expression of DTL also exhibited the decreased invasion (G and g) and migration ((H,I) and (h,i)) of cancer cells. ((J,K) and (j,k)) Depleted DTL suppressed EMT by western blot (J and j) and immunofluorescence staining (K and k). *P < 0.05, ***P < 0.001, ****P < 0.0001.
