Fig. 4 | Scientific Reports

Fig. 4

From: Suppression of JNK pathway protects neurons from oxidative injury via attenuating parthanatos in glutamate-treated HT22 neurons

Fig. 4

Effects of reactive oxygen species (ROS) on parthanatos at 12 h in glutamate-treated HT22 neurons. (a) Fluorescence intensity of DCFH-DA in HT22 neurons was enhanced at the indicated time points (6, 8 and 12 h); this trend was counteracted by NAC pre-treatment. (b) Pre-treatment with NAC decreased glutamate-induced DNA injury in HT22 neurons, as shown by 8-OHdG expression levels. (c) Western blot showed that NAC pre-treatment decreased PARP-1 and PAR expression levels and (d) increased cytoplasmic AIF expression but decreased nuclear AIF expression. Actin and Histone were used as cytoplasmic and nuclear loading controls, respectively. Data are expressed as mean ± standard deviation (SD; *p < 0.05, **p < 0.01).

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