Figure 8
From: Silencing CCT3 induces ferroptosis through the NOD1-NF-κB signaling pathway in bladder cancer
Silencing CCT3 induced ferroptosis to suppress BCa progression in mice by inhibiting the NOD1-NF-κB pathway. (A) Body weight of mice. (B) Tumor weight. (C) The image of tumors from different groups. (D) The relative mRNA expression level of CCT3 was determined by RT-qPCR. (E) The results of hematoxylin-eosin staining (100×, scale bars = 200 μm). (F) The results of Ki67 staining (100×, scale bars = 200 μm). (G) The relative protein expression level of CCT3 was detected by western blotting. (H) Content detection of MDA, GSH, and SOD by corresponding ELISA kits. (I) The iron assay kits were applied to detect the Fe2+ levels. (J) The protein expression levels of NOD1, IκBα, p-IκBα, SLC7A11, and GPX4. ***p < 0.001 vs. LV-NC group; ###p < 0.001, ##p < 0.01, #p < 0.05 vs. LV-CCT3 group.
