Fig. 3

Intraoperative data. Comparison of (A) peripheral and (B) portal cfDNA (copies/mL, mean, SD) and ctDNA (copies/mL, mean, SD) levels before and after tissue mobilization. (C) KRAS mutation detection in tumor specimen (“Tumor”) and in peripheral and/or portal vein, before and/or after tissue mobilization during surgery (“Peroperative ctDNA”). Recurrence-free survival (D) and Overall survival (E) according to evolution of ctDNA after mobilization comparing to before tissue mobilization. **** p < 0.0001, ** p < 0.01, * p < 0.05. ctDNA: Circulating tumor DNA, cfDNA: Circulating-Free DNA, NS: not significant, SD: Standard deviation.