Fig. 4

Normalization with the different housekeeping gene candidates on VEGF and TNF-α expression levels in mice’s tumor, liver, and lung with or without 4T1 breast carcinoma. The blue line highlights the gene expression normalized with the most stable gene; the Red line highlights the gene expression normalized with the least stable gene. The Mann-Whitney test was used to analyze statistical differences between the control group (n = 8 and the 4T1 group (n = 8). Significant differences are represented by *p < 0.05; **p < 0.01; ***p < 0.001.