Table 1 Information about primers designed in this study include sequence, amplicon size, slope values, efficiency values (E%), correlation coefficients (R2), melting temperature (°C) and accession number. F: Forward; R: reverse.
Gene | Sequence | Amplicon length (Pb) | Slope | E (%) | R2 | Tm (°C) | NCBI accession number |
|---|---|---|---|---|---|---|---|
GUSB | F: TCCAAGGGGTCAATAAGCAC R: GGGATAGTGGCTGGTACGAA | 128 | -3.10 | 110.17 | 0.997 | 82.64 | NM_010368.2 |
PGK1 | F: ATGGATGAGGTGGTGAAAGC R: CAGTGCTCACATGGCTGACT | 118 | -3.44 | 95.30 | 1.000 | 81.81 | NM_008828.3 |
CCSER2 | F: GAATCTCACCCCGATGCTTA R: ACACAAAACCTGGCCAGAAC | 115 | -3.16 | 107.23 | 0.968 | 79.38 | XM_036158858.1 |
SYMPK | F: AAGCCATCTACACCCCTGTG R: GCTCCTCCTTGCACTGTTTC | 135 | -3.33 | 99.96 | 1.000 | 85.99 | NM_026605.3 |
ANKRD17 | F: CCCTCACAAGACTCCAGCTC R: CGAAGGTAGCTGGACAGAGG | 106 | -3.07 | 111.71 | 0.999 | 80.74 | XM_006535287.5 |
VEGF | F: CCTTCGTCCTCTCCTTACCC R: AAGCCACTCACACACACAGC | 117 | -3.27 | 105.82 | 0.984 | 83.20 | NM_001025250.3 |
TNF-α | F: TATGGCTCAGGGTCCAACTC R: CTCCCTTTGCAGAACTCAGG | 174 | -3.32 | 100.08 | 0.980 | 83.23 | NM_013693.3 |
