Fig. 2 | Scientific Reports

Fig. 2

From: LncRNA BRE-AS1 regulates the JAK2/STAT3-mediated inflammatory activation via the miR-30b-5p/SOC3 axis in THP-1 cells

Fig. 2

Reduction of BRE-AS1 Enhances IL-6 and IL-1β Expression Without Affecting TNF-α. A The effectiveness of the knockdown was evaluated by real-time PCR 24 h after transfecting THP-1 cells with scramble or BRE-AS1 siRNA (n = 3). B–D Following LPS stimulation (1000 ng/ml), mRNA levels of cytokines were quantified using real-time PCR (n = 3). E, F Cytokine secretion levels were measured by ELISA after LPS stimulation for 24 h at specified concentrations for IL-6 E and at indicated durations for TNF-α with LPS (1000 ng/ml) F (n = 4). **p < 0.01; ***p < 0.001. Error bars represent SEM.

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