Fig. 5 | Scientific Reports

Fig. 5

From: LncRNA BRE-AS1 regulates the JAK2/STAT3-mediated inflammatory activation via the miR-30b-5p/SOC3 axis in THP-1 cells

Fig. 5

BRE-AS1 Modulation of SOCS3 mRNA via miR-30b-5p Interaction. A–C THP-1 cells were transfected with a miR-30b-5p inhibitor alongside BRE-AS1 siRNA for 24 h before LPS stimulation (1000 ng/ml). A, B mRNA levels of SOCS3 and IL-6 were quantified using real-time PCR (n = 4). C Protein levels within the SOCS3/JAK2/STAT3 pathway were analyzed by Western blot. D–G THP-1 cells underwent transfection with miR-30b-5p mimic and mimic control for 24 h. D–F The mRNA expression of SOCS3, IL-6, and IL-1β was assessed using real-time PCR (n = 3). (G) Protein levels of the SOCS3/JAK2/STAT3 pathway were evaluated by Western blot. *p < 0.05; **p < 0.01; ***p < 0.001. Error bars represent SEM.

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