Table 1 Central composite design for two-variables and three-levels and observed responses under PEF method extraction.

From: Comparison of microwave and pulsed electric field methods on extracting antioxidant compounds from Arvaneh plant (Hymenocrater platystegius Rech. F)

Exp. No

Variables

Responses

A

B

Y1

Y2

Y3

Y4

Y5

Intensity (kV/cm)

Pulse

Yield (%)

TPC (mg Gallic acid/g)

Flavonoid (mg quercetin/g)

DPPH (%)

FRAP (µmol Iron/ml)

1

3.25 (0)

45 (0)

19.18

309.21

2599.39

98.45

221.28

2

6.25 (+ 1)

10 (-1)

14.42

290.38

2590.12

89.82

211.76

3

0.25 (-1)

80 (+ 1)

15.48

291.61

2582.53

91.41

213.47

4

6.25 (+ 1)

80 (+ 1)

21.29

268.38

2583.11

81.31

203.35

5

3.25 (0)

45 (0)

19.32

308.25

2600.17

97.09

218.19

6

3.25 (0)

10 (-1)

17.08

296.37

2594.38

88.41

210.38

7

0.25 (-1)

10 (-1)

12.34

286.64

2586.31

71.58

193.63

8

6.25 (+ 1)

45 (0)

18.37

290.28

2596.38

95.27

217.11

9

3.25 (0)

45 (0)

18.17

305.17

2598.28

95.59

217.65

10

3.25 (0)

80 (+ 1)

21.39

291.52

2589.42

92.31

214.28

11

3.25 (0)

45 (0)

18.31

306.35

2598.32

96.21

219.11

12

3.25 (0)

45 (0)

20.24

305.26

2599.61

99.11

220.78

13

0.25 (-1)

45 (0)

14.42

291.34

2591.22

89.31

211.47

  1. TPC: Total Phenolic Compound, DPPH: scavenging activity of DPPH, Frap: Ferric Reducing Antioxidant Power.
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