Table 2 Central composite design for two-variables and three-levels and observed responses under MW-assisted extraction on bioactive compounds and antioxidant activity of APE.

From: Comparison of microwave and pulsed electric field methods on extracting antioxidant compounds from Arvaneh plant (Hymenocrater platystegius Rech. F)

Exp. No

Variables

Responses

A

B

Y1

Y2

Y3

Y4

Y5

Power (W)

Time (S)

Yield (%)

TPC (mg Gallic acid/g)

Flavonoid (mg quercetin/g)

DPPH (%)

FRAP (µmol Iron/ml)

1

900 (+ 1)

90 (-1)

4.37

90.64

1590.27

79.86

113.81

2

540 (0)

180 (0)

9.26

106.17

1599.28

90.96

119.07

3

900 (+ 1)

270 (+ 1)

11.47

58.61

1575.03

76.26

95.24

4

540 (0)

180 (0)

8.29

104.31

1597.34

93.25

121.78

5

540 (0)

180 (0)

10.32

105.11

1598.78

91.96

118.91

6

540 (0)

180 (0)

9.26

107.28

1599.24

92.05

120.24

7

540 (0)

180 (0)

8.36

108.18

1599.32

90.25

118.20

8

540 (0)

270 (+ 1)

10.39

87.37

1587.31

82.65

114.22

9

180 (-1)

270 (+ 1)

5.26

89.38

1585.64

80.36

112.55

10

900 (+ 1)

180 (0)

9.24

85.28

1588.11

88.28

108.02

11

180 (-1)

90 (-1)

2.79

66.38

1584.29

70.43

104.7

12

540 (0)

90 (-1)

7.09

97.34

1595.17

80.11

115.21

13

180 (-1)

180 (0)

3.38

92.52

1590.27

81.34

110.54

  1. TPC: Total Phenolic Compound, DPPH: scavenging activity of DPPH, Frap: Ferric Reducing Antioxidant Power.