Fig. 3
Knockdown of CYFIP2 enhances CDDP sensitivity in ESCC cells. (A,B) Colony formation assays confirmed that CDDP-resistant cells exhibited faster cloning rates (IC50 vs NC). However, when CYFIP2 was knockdown, the cloning of ESCC cells was significantly inhibited (IC50 + sh01/04 vs IC50). (C,D) EdU assays, with DAPI serving as a staining control, revealed enhanced proliferative rates in resistant TE-1 and EC109 cell lines (IC50). In addition, CYFIP2 silencing (IC50 + sh01/04) resulted in decreased proliferation. The EdU images are presented with a scale bar of 100 μm. (E,F) The level of ROS in cells was measured by fluorescent probe detection. Each photo has a scale 200 μm in the lower right corner. Error bars represent the mean ± SD for independent repeated data, and “*”, “**”, “***”, and “****” represent p-value < 0.05, p-value < 0.01, p-value < 0.001, and p-value < 0.0001, respectively.
