Fig. 4
Inhibition of CYFIP2 downregulates autophagy in ESCC cells. (A,B) The accumulation of LC3 on autophagosomes was observed in cells transfected with GFP-LC3 under fluorescence microscopy (scale bar: 20 μm). (C,D) Cells transfected with mRGFP-GFP-LC3 were detected under fluorescence microscopy to dynamically analyze the fusion of autophagosomes and lysosomes (scale bar: 20 μm). (E) Electron microscopy was used to compare and analyze autophagosome formation in cells. Scale bar (up): 5 μm; Scale bar (down): 2 μm. (F,G) Beclin1 and LC3I/II were detected by western blot to evaluate the autophagy level in cells. Error bars represent the mean ± SD for independent experiments, and “*”, “**”, and “***” represent p-value < 0.05, p-value < 0.01, and p-value < 0.001, respectively.
